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KMID : 1005220130280030257
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Journal of Embryo Transfer
2013 Volume.28 No. 3 p.257 ~ p.263
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Expression of Plasminogen Activators in Uterine Epithelial Cells of Pre-ovulatory Phase in Pigs
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Hwangbo Yong
Lee Sang-Hee
Cha Hye-Jin
Song Eun-Ji
Lee Seung-Tae
Lee Eun-Song
Cheong Hee-Tae
Yang Boo-Keun
Park Choon-Keun
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Abstract
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The endometrium undergoes a cyclic growth and tissue remodeling as changes of epithelial cells, and plasminogen activators (PAs) are related to endometrium tissue remodeling. This study was to evulate expression of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in porcine uterine epithelial cells. In results, the uPA and tPA were expressed in uterine tissue, epithelium and secretory glands in porcine endometrial cell. In addition, the uPA and tPA were expressed in cultured epithelial cells, and it were mainly expressed in cytoplasm. In porcine uterine tissue and epithelial cells, uPA activity was higher than activity in tPA. In PAs mRNA expression levels, uPA mRNA level was significantly higher than tPA mRNA level (P<0.05). The fluorescence intensity of uPA protein was also higher than fluorescence intensity of tPA protein, and uPA protein expression was significantly higher than in tPA protein expression (P<0.05). Therefore, we suggest that a physiological function in porcine uterine epithelial cells should be more influenced by uPA than in tPA during pre-ovulatory phase.
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KEYWORD
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porcine uterus , endometrium , epithelial cells , urokinase-type PA , tissue-type PA
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